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As a novel GLS1 inhibitor, the recent reported compound 19 (C19) is thought to have a similar or greater ability to inhibit anti-CD3/CD28-induced CD4 + T cell proliferation and cytokine production by directly binding to the GAC outside the active site compared to BPTES (55)

Calu-3 cells (human lung adenocarcinoma cells) and HEp-2 cells (human epidermoid cancer cells) were maintained at 37 C with 5% CO 2 in Dulbeccos Modified Eagle Medium (DMEM) containing 10% FCS, GlutaMax, and 100 g/mL penicillin-streptomycin

Saeedi P, Salpea P, Karuranga S, Petersohn I, Malanda B, Gregg EW, et al

But what is this molecule called glutathione, and why are many now seeking the IV route to treat it

Science 330, 841845