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The tissue sections were briefly air-dried and then incubated in the dark with cy3-goat anti-mouse (1:300

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Hritani R, Al Rifai M, Mehta A, German C

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The reaction mixture for each well consisted of 5 L of master mix, 0.5 L of each forward and reverse primer (10 M) and 1 L of 1 in 10 dilution of cDNA