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doi: 10.1111/j.1360-0443.2011.03402.x [DOI] [PMC free article] [PubMed] [Google Scholar] 42.Elton-Marshall T, Fong GT, Yong HH, et al

A 25 L reaction mixture was prepared, comprising 12.5 L PCR Master Mix (2X) (Thermo Scientific), 0.8 L of 10 mM forward primer, 0.8 L of 10 mM reverse primer, 2 L MT-DNA (20 ng/L), and 8.9 L nuclease-free water

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