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Common lab protocols involve reconstitution in bacteriostatic water and subcutaneous injection in animal models using calibrated syringes to achieve precise dosing

Oral versions such as capsules or troches are more convenient, but absorption through the digestive tract is far less consistent, so the amount that actually reaches circulation can vary widely

However, outliers were analyzed for these correlations using the interquartile range (IQR) method

The use of methotrexate may additionally invalidate diagnostic assays for folic acid and vitamin B12

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Standard dosing approach The most commonly cited dosing range is 50 to 150mg per day, typically divided into one to three doses