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These limitations are addressed in fed-batch and continuous exchange (also referred to as semicontinuous) setups in which additional template, substrates, and/or energy sources are provided during the reaction, 29 increasing reaction longevity and/or yield

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CD31 + SOX17 + artery area, length, GFP + area and nuclear density were quantified using Photoshop and ImageJ softwares

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Methods Sample preparation for ice nucleation experiments Aqueous solutions were prepared at concentrations of 5 mg mL 1 , 0.5 mg mL 1 , and 5 10 4 mg mL 1 using ultrapure water (HPLC water, Sigma-Aldrich) and commercially available compounds, which were obtained from Sigma-Aldrich unless otherwise noted

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