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With regard to long-term treatment, the parents were very reluctant to tolerate routine IM injections of OHCbl in their child

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Therefore, neuronal subsets (iSPNs) from mouse striatal tissues of mutants and controls (here called iSPN + mut and iSPN + wt populations, respectively) were genetically labelled in vivo with a fluorescent marker using Rosa26-Ai9-tdTomato strain 21 and purified by fluorescence-activated cell sorting

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