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This reaction forms an unusual -peptide bond between the -carboxyl group of glutamate and the amino group of cysteine

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The final absorbance was calculated by subtracting absorbance of 595 nm from that of 570 nm

Here, the lock is the immobilized ligand on the chromatographic support, which is opened by the specific site on the target molecules, or protein, which serves as the key

& Orecchia, R