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Both assays were performed with 0.1 mM NADPH and 1 U ml 1 GR

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The artPTLs were prepared for PTL ACair and PTL DC , whose antimicrobial effects were evaluated as the most promising

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It also shows that site-directed mutagenesis of Hh amino acids corresponding to Ptc-binding K46 and K88 ( Drosophila ortholog) or adjacent residues R238 and R239 do not affect Hh binding to Ptc, as this would have resulted in smaller eyes