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Of these, 50 had hypothyroidism due to Hashimotos thyroiditis (HT group), 30 had nonautoimmune hypothyroidism (NAIH group), and 36 were healthy controls (control group)

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Step two: gamma-glutamylcysteine and glycine combine under glutathione synthetase (GS) to form glutathione (GSH)

Indeed, when GSAAT is reduced with gradually increasing amounts of DTT prior to incubation with NEM, either one Cys (Band 1, with 0.1 mM DTT) or no Cys residue of GSAAT is accessible for mPEG binding (with DTT 1 mM) ( Figure 2C )

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