Firstly, chloroplasts actively down-regulate 53 and reduce the processing of RNA encoding genes in photosynthesis-related apparatus 54 , control the routes of chloroplast protein import 55 , phosphorylate chloroplast proteins to inhibit electron transport rate 56,57 , reduce cyclic electron flow (CEF), reduce NPQ, and degrade PSII 15,58 , and finally inhibit photosynthesis to reduce energy obtained by other cellular activities and pathogens and prioritize energy investments
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(2022) 55:982997.e8
The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added
