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Toxic megacolontotal or segmental nonobstructive dilatation of the colon with systemic toxicity in patients with IBD or infectious colitis Laboratory values of acute phase reactants, including the erythrocyte sedimentation rate and C- reactive protein, can be increased but may also be normal in mildly active disease

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Subsequently, cells were washed in Tris Buffered Saline (TBS) and permeabilized for 25 min in 2% Triton X-100 in TBS, depending on the antibody

Two injections of the same drug at the same site don't qualify