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Total cells from the BAL were centrifuged and counted using Countess II Automated Cell Counter (Invitrogen, Carlsbad, CA) as previously described ( Morphometric assessment In order to determine the effects of ENDS exposure on the development of emphysema, we did a morphometric assessment according to the previous protocol ( Immunohistochemical, immunofluorescent, and histology analysis The mouse lungs were inflated with 1% low melting-point agarose to 25 cm of fixative pressure and then fixed with 4% neutral buffered formalin for 48 hours
ISSN 1879-1646
High doses of THC the main psychoactive component can increase risks of mental health complications and dependency
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Children and unborn fetuses are likely more susceptible to the dangerous effects of cannabis smoke, so its recommended that pregnant women, and women trying to become pregnant, avoid use of cannabis products
