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A prespecified analysis of this trial showed that a greater weight loss was associated with greater improvements in HF-related symptoms and physical limitations, regardless of patients age, gender and baseline body weight 89

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RNA extraction, cDNA conversion, and qPCR RNA from 21NT, BT474, IBR.3, and HMEC was extracted, and mRNA gene expression analysis was performed (primers listed in Table 1) using qPCR as described previously (Al-dulaimi et al 2024)