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In the long-term toxicity experiment, continuous injection of AOD 9604 (10 mg/kg) for 8 weeks did not cause abnormal liver and kidney functions or fluctuations in blood glucose, providing a safety basis for its further research and application

Its best to be hydrated and relaxed, and to wear clothing that allows easy access to the injection site

Mishra R, Upadhyay A (2025) An update on mammalian and non-mammalian animal models for biomarker development in neurodegenerative disorders

Prolotherapy: Which is Better

During this phase, the dosage is reduced to 2-4 mg per week

Many patients report a crisp surge in mental alertness, clarity, and physical stamina within 12 to 24 hours post-injection