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The animals were placed in the stereotaxic device (David Kopf Instruments, Tujunga, CA, USA) and holes were drilled in the frontal, parietal, and occipital regions of the skull at the following locations: 2 mm from Bregma, 2 mm lateral to the midline (ipsilateral), and 2 mm posterior to Lambda (reference electrode)

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The proper process involves cleaning rubber stoppers, injecting water slowly down the vial wall, letting peptide dissolve naturally without shaking, and immediately refrigerating after use

Thioredoxin glutathione reductase-dependent redox networks in platyhelminth parasites

Butyrate is the short-chain fatty acid that fuels colonocytes and drives peristaltic rhythm