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GSH can then be synthesized de novo in a two-step ATP-requiring enzymatic process utilizing glutamate cysteine ligase (GCL) and glutathione synthetase (GS) (Figure 2) [83,87]

At baseline, trained nurse interviewers administered a structured questionnaire to each subject to collect comprehensive information on demographics, occupation, lifestyle habits, medical history, usage of medication and dietary supplements, alcohol consumption, and past cigarette smoking history

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