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Protein (50 g) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (412%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

279 In Escherichia coli , an elevated ratio of NADH to NAD + inhibits pyruvate dehydrogenase (PDH) activity, impeding the formation of acetyl-CoA, as the conversion of pyruvate to acetyl-CoA relies on PDH

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doi: 10.1080/1828051X.2018.1456978 34