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After three washes with TBST, membranes were incubated with goat anti-rabbit or mouse secondary antibody (1:5000) at room temperature for 1 h, followed by three 10-min washes
However, no major adverse effects have been reported till now
Figure 2B shows that using the dbEMT, an epithelial-mesenchymal transition (EMT) associated gene resource (35), CR DEGs were classified as: oncogenic EMT-related genes (CR upregulated: Aldh1a1, Aldh1a7, Srebf1