"Finding that interesting loop later led to our understanding of the sensing mechanism," Birsoy says
Chen S, Peng Y, Zhang H, Zou Y (2023) Relationship between thyroid function and dietary inflammatory index in Hashimoto thyroiditis patients
Will continue to use Vitastir
So, I was skeptical before coming here
The overnight culture (2030 ml) was added to 1,000 ml of 2YT medium (containing 100 g/ml carbenicillin and 50 g/ml kanamycin) up to an optical density at 600 nm (OD 600 ) = 0.1 and grown at 37C until OD 600 = 0.6 before induction with 1 mM isopropyl--D-1- thiogalactopyranoside
For glucose measurements, after sample drying with nitrogen gas, dried lysates were mixed with pyridine:acetic anhydride (1:2) solution and incubated at 60C for 30 min