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In case blood samples were further processed for a scRNA-seq, a density gradient centrifugation was then conducted using Histopaque 1077/1119 (Sigma-Aldrich) following the manufacturers protocol

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Depending on the nature of the neurons depolarization, NMDA-Rs can both strengthen synapses, through long-term potentiation (LTP) [32, 33], and weaken synapses, through long-term depression (LTD) [34]

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