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However, these uncontrolled observations require validation through prospective, controlled clinical trials before establishing standard treatment durations for specific clinical indications

Subcutaneous and intramuscular injections use different tissue targets and require different training

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After appropriate treatments, cells were washed in PBS and then lysed in 100 to 150 l of buffer (100 mM NaPO 4 , 1 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5) containing 0.1% Triton X-100 and frozen at -80C until analysis

This safeguard is especially important for topical formulations, where repeated application over time could otherwise lead to gradual accumulation