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For the estimation of the total AsA concentration, the oxidized fraction was reduced by 0.1 M dithiothreitol

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The present work compares, for the first time, whether the season-dependent effects of heat stress (winter, spring, summer, and autumn) are replicated in an in vitro model, which is crucial for a deeper understanding of the physiological mechanisms underlying oocyte damage

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On a 1 mL insulin syringe (100 units), every 20 units equals 1 mg