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The intramuscular acidic saline injection model is another commonly used model that uses repeated injections of acidic saline into the gastrocnemius muscle to develop widespread pain 34,35

Unlike an overdose in humans, liver damage is rarely the cause of death, instead, methaemoglobin formation and the production of Heinz bodies in RBCs inhibit oxygen transport by the blood, causing asphyxiation (methaemoglobinaemia and haemolytic anaemia)

Other possible side effects: nausea, vomiting, diarrhea, abdominal pain, fishy body odor

Injections are usually given as hydroxocobalamin or cyanocobalamin and are very effective in increasing blood B12 levels and preventing deficiency