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HS-His3MX6-pTEF1-HS and HS-pGSH1-URA3-GFP-tADH1-HS fragments amplified from the relevant pRS315 constructs (described above) were each used to transform both strains BY4741 and BY4742, to replace pGSH1 with pTEF1 and inserting URA3-GFP-tADH1 in the URA3 locus, creating pgsh1 D:: His3MX6-pTEF1

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