All cell lines tested negative for mycoplasma contamination using a MycoAlert Mycoplasma Detection kit (Lonza, LT07-418)
It helps maintain the structure and activity of important proteins and enzymes in the body, such as glutathione
Glutathione-S-transferase mu 2 ( GSTM2 ), as a member of phase II drug metabolizing enzymes (DMEs), regulates cellular antioxidant and detoxificant
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Cells were also treated with 80 mM Dynasore (120287002, Sigma) or vehicle control for 30 min with or without Sema4D-Fc-647 (2 h, 2 g/ml), then fixed and stained with DAPI
Effects of HT and exogenous GSH on gene expression related to photosynthesis and antioxidant enzymes The expression level of all the Calvin cycle enzyme genes investigated here were significantly up-regulated during the HT stress under the HT+GSH treatment compared to the HT treatment (except for fructose-1,6-bisphosphatase ( FBPase ) for which the increase was not significant) (Fig