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Material and methods Cell culture Primary neurons were obtained from the triple transgenic mouse model of AD (3xTg-AD) with human APP (SWE), PS1 (M146V), and Tau (P301L) transgenes [18] in three different age ranges: young (26 months), middle (811 months) and old (1728 months), around the median lifespan

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Salcedo-Arellano et al., 2021)