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#4 Environmental Toxins Exposure to environmental pollutants, heavy metals (such as lead, mercury, and arsenic), pesticides, and other toxins found in air, water, and food sources can impair glutathione synthesis and utilization

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However, one of the primary drawbacks of OOAC models is the limited number of cells that can be cultured within these small devices that often are too minute for traditional biochemical detection methods such as Western blotting, gel electrophoresis, and colorimetric detection [225]

Notably, a recent study identified L-lactylation as the primary form induced by hypoxia in tumors, linking it to hypoxia markers and tumor malignancy

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