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Another biosynthetic capability of plant systems is the production of terminal polysialic acid (polySia) glycoproteins, which can address challenges associated with the existing polySia conjugation process, such as the need for numerous fermentations, product purification, and in vitro chemical interactions ( PolySia plays multiple roles in various biological processes, such as cell regeneration, various immunological processes, and brain development Margolin et al., 2023) and (Phoolcharoen et al., 2023) suggests that manufacturing these molecules in plant systems can offer solutions to current challenges such as unequal vaccine distribution, high costs per dose, and the need for cold chain infrastructure, as discussed by ( Although plant glycoengineering has advanced significantly over the past 15 years, there are still several targets that need to be designed to minimize N and O-glycan variability and prevent potentially immunogenic glycan epitopes

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