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Cells were washed in staining buffer (5 mM CaCl 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

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The science is clear: glutathione (GSH) is your bodys master antioxidant-produced in every cell, it protects against oxidative stress, neutralizes toxins, supports immune function, and regulates inflammation