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nRFP-labelled portal fibroblast cultures (passage 512) grown in DMEM +++ with 20% FBS (Merck/Sigma, F7524) were washed with PBS and dissociated to single cells by incubation with 1 TrypLE for 6 min at 37 C

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Preliminary findings suggest that one of the Fragment 176-191 test groups might have experienced a noticeable reduction in body weight, estimated at approximately 5.7 pounds

Development of an enzyme immunoassay for delta sleep-inducing peptide (DSIP) and its use in the determination of the metabolic clearance rate of DSIP administered to dogs