Furthermore, the current analysis revealed that e-cigarette use among city and village residents was significantly greater than that among camp residents
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Then, they were incubated with primary antibodies at 4 C for overnight and stained with FITC- (A0562, beyotime) and Coralite594-conjugated secondary antibody (SA00013-4, Proteintech) at room temperature for 1 h, after which nuclear were stained with DAPI (F6057, sigma)
The researchers also looked at the websites claims about e-cigarettes, noting whether they suggested that e-cigarettes were less harmful than conventional cigarettes, or cheaper than regular cigarettes, or a good way to quit smoking
