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The positions of the probes and the free energy values were exported in a PDB file and visualized in Pymol 74

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Psefteli PM, Kitscha P, Vizcay G, Fleck R, Chapple SJ, Mann GE, Fowler M, Siow RC

This is the vial size most researchers start with, and many continue using it through the 4mg escalation phase

Subsequently, TB was removed and replaced with 50 L of TB-containing compounds at different concentrations or probe substrates with or without inhibitors, and the cells were incubated at 37C for 10 min

Eating slower, choosing softer foods initially, and staying hydrated can ease this transition