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Validation of the transcriptomic analysis results via immunoblotting From the DEGs identified in the ovarian transcriptome analysis, we selected DEGs that are related to mitochondrial function, energy metabolism, and inflammatory and immune pathways

For example, increased methylation of the PGR gene promoter has been associated with reduced progesterone receptor expression during luteal regression

The antidepressant effects of extracts of parts of plants such as the Silybum marianum seed have also been reported[26]

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This metabolic imbalance contributes to increased lipid peroxidation, as indicated by high malondialdehyde (MDA) levels (Chauhan et al., 2004), and is exacerbated by a concomitant reduction in antioxidant defenses