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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Most research has focused on taking vitamin D orally

After mixing, the optical density (OD) for each well was measured using a microplate reader

Science, 219 , 13411343

Nevertheless, researchers are actively investigating ipamorelin for body composition due to its ability to increase HGH levels

Safety and tolerability (clinical evidence): Comprehensive controlled human safety data are limited