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These findings were recently confirmed and extended by Wilding et al

Signs and Symptoms of Vitamin B12 Deficiency Vitamin B12 deficiency is characterized by megaloblastic anemia, fatigue, weakness, constipation, loss of appetite, and weight loss

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For example, in vivo studies have shown that inhibition of the pro-BDNF to mBDNF conversion leads to autism spectrum disorder (ASD)-like phenotypes 27 , suggesting that the pro-BDNF/BDNF ratio could serve as a potential biomarker for this disorder