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The membrane was washed three times in PBS prior to 1 hour of incubation in PBS containing goat anti-mouse IgG conjugated with horseradish peroxidase

[21] When treated with cyanocobalamin, patients with Leber's disease may develop serious optic atrophy, possibly leading to blindness, based on few case reports

Seek immediate medical attention if you develop rash, difficulty breathing, or facial swelling following injection

It is typically given via intramuscular or intravenous administration under medical supervision

The Real Answer: It Depends on Your Protocol Here's the truth that often gets lost in online forums and confusing guides