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qPCR was performed according to the protocol consisting of 40 cycles: polymerase activation and DNA denaturation 95 C (3 min), denaturation 95 C (15 s), annealing 60.5 C (15 s), extension and plate reading at 72 C (40 s)

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This makes it a highly valuable adjunct therapy for managing the unpredictable, systemic flare-ups associated with Mast Cell Activation Syndrome (MCAS) and generalized histamine intolerance

Rossiter ND, Chapman P, Haywood IA

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