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The crude product was purified by a silica gel column chromatography using hexane and dichloromethane mixture (10:1 v/v) as eluent and obtained as yellow powder in 74% yield

Together, they provide synergistic stimulation of growth hormone release, making this vial a powerful research compound for studying growth hormone dynamics, IGF-1 signaling, metabolic regulation, and endocrine system function

4-Octyl Itaconate also showed effects in reducing chondrocyte degeneration, oxidative stress, and ferroptosis by inhibiting GPX4 methylation (Pan et al., 2024)