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Participants were divided into two groups based on the age they started smoking (less than 20 years old and 20 years or older)

After transfection, 1 mL of 10% FBS human macrophage cell culture medium was added to each cuvette

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Due to the constant self-regeneration after peeling and the longevity as well as robustness of the cork oak, the raw material cork is almost inexhaustible and thus of a uniquely sustainable character

AO-red spermatozoa are scored as AO positive (AO+) and the percentage of AO + sperm is calculated