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Cells were maintained in DMEM (Gibco, 50-003-PB) supplemented with 3.7 g l 1 sodium bicarbonate (Sigma-Aldrich, S6297), 10% fetal bovine serum (FBS) (Gibco, 26140079) and 1% penicillinstreptomycin solution (Sigma-Aldrich, P4333)

Noise level : Choose a machine with a low noise level 54dB (A) to avoid disturbing daily activities, especially in the bedroom

The sectors with the highest percentages of Black workers are protective services (45% Black) and transportation workers (35% Black)

Get extra sleep: Withdrawal symptoms from quitting smoking include difficulty sleeping and tiredness

This integration ensures real-time coordination between visual inspection, optimization decisions, and machine control, thereby enabling adaptive, scalable, and reliable quality control in industrial cigarette manufacturing environments