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The capillary temperature and capillary voltage were maintained at 320 C and 3.5 kV, respectively

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Swirl gently: Once diluent is added, swirl the vial gently until the powder is fully dissolved

While singly-housed animals may experience stress that impacts metabolism, body weight, and adiposity 65,66,67,68,69 , the singly-housed animals in this study were treated identically (to the best of the experimenters ability) to minimize and equalize stress across all animals and reduce confounding variables

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