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MPC1-deficient C2C12 cells were generated by CRISPR and cultured as described (Oonthonpan, Rauckhorst, Gray, Boutron, & Taylor, 2019)

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The publication of high-quality mechanistic studies, coupled with small human trials showing functional endocrine and immune effects , has elevated the peptide from fringe curiosity to a serious candidate in the longevity toolkit

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