Treatment of Arabidopsis seedlings with 5 mM H 2 O 2 increased GCL activity and resulted in a shift from the less active reduced form to the more active oxidized form, as determined by activity assays and immunoblot analysis (Hicks et al., Conservation of the disulfide linkage that mediates the monomer/dimer transition of GCL occurs across the plant kingdom (Gromes et al., Nicotiana tabacum ), Agrobacterium tumefaciens (a-proteobacteria), and Xanthomonas campestris (-proteobacteria) showed that the two proteobacterial GCL remain monomeric during redox changes, even though the disulfide is retained
The mechanistic logic is solid even though the human study has not been done Reduce exposure where you can: do not heat food in plastic containers, switch to glass or stainless steel
10.1186/s41983-022-00452-2 Egypt J Neurol Psychiatry Neurosurg
ROS staining, fluorescence quantification of CellROX, and ion leakage assays For ROS staining of N
Xia N, Daiber A, Forstermann U, Li H
Smith, C